cat 55135 1 ap Search Results


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Cat 55135 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech α sma
(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP <t>and</t> <t>α-SMA.</t> (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate <t>of</t> <t>α-SMA</t> and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.
α Sma, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Wuhan Sanying Biotechnology primary antibodies against α-sma
(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP <t>and</t> <t>α-SMA.</t> (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate <t>of</t> <t>α-SMA</t> and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.
Primary Antibodies Against α Sma, supplied by Wuhan Sanying Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP <t>and</t> <t>α-SMA.</t> (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate <t>of</t> <t>α-SMA</t> and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.
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Proteintech 55135 1 ap
(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP <t>and</t> <t>α-SMA.</t> (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate <t>of</t> <t>α-SMA</t> and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.
55135 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech rabbit anti-α-sma antibody
(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP <t>and</t> <t>α-SMA.</t> (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate <t>of</t> <t>α-SMA</t> and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.
Rabbit Anti α Sma Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioss acta2 (3f9) monoclonal antibody
(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP <t>and</t> <t>α-SMA.</t> (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate <t>of</t> <t>α-SMA</t> and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.
Acta2 (3f9) Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP and α-SMA. (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate of α-SMA and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.

Journal: medRxiv

Article Title: Schizandrin A suppressed pancreatic cancer proliferation and cancer associated fibroblast activation via inhibiting the COX-2/ALOX5 shunting

doi: 10.1101/2024.10.09.24315180

Figure Lengend Snippet: (A) EPIC algorithm was used to analyze immune cells in PC tissues from the TCGA cohort. (B) The positive association between COX-2/ALOX5 expression and CAF levels in PC tissues from TCGA cohort. (C) PC tissues with high COX-2 expression exhibited up-regulated FAP expression. (D) PC tissues with high ALOX5 expression exhibited up-regulated FAP expression. (E) Conditioned medium (CM) derived from PC cells treated with both DMSO or schizandrin A was collected and utilized to treat normal fibroblasts (NFs) with a 90% vimentin positive rate. (F) Immunofluorescence analysis indicated that NFs cells cultured with CM from schizandrin A treatment-PC cells exhibited lower FAP and α-SMA. (G) Mode pattern of the subcutaneous tumor formation test. (H) Tumors derived from NFs and SW1990 cells after schizandrin A demonstrated reduced tumor volume and weight. (I) Immunohistochemical analysis of tumor tissues revealed a lower expression rate of α-SMA and FAP in tumors derived from NFs and SW1990 cells treated with schizandrin A compared to those treated with DMSO. **, P<0.01.

Article Snippet: Subsequent procedures involved additional washing with PBS, incubation with the suitable primary antibody including α-SMA (1:50, Cat no. 55135-1-AP, Proteintech, Wuhan, China), and FAP (1:100, Cat no. #66562, CST, USA) in a humidified chamber at 4°C, followed by overnight incubation, washing, and incubation with the appropriate secondary antibody for 2 hours at room temperature.

Techniques: Expressing, Derivative Assay, Immunofluorescence, Cell Culture, Immunohistochemical staining